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Cloning of Human Interleukin-17 and Its Expression in E. coli / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy ; (6)1994.
Article in Chinese | WPRIM | ID: wpr-581839
ABSTRACT
Interleukin-17 (IL-17) is a newly found cytokine secreted by activated T lymphocytes. From preliminary study, IL-17 was found to play a role in the relationship between T lymphocytes and hematopoiesis, but many of its characteristics remain unknown up to now. To investigate its biological functions and related mechanisms, we cloned the complete coding region of human IL-17(hIL-17) from activated human peripheral blood mononuclear cells (PBMC) by RT-PCR and constructed an integrative vector pLCM182-hIL-17 for cloning, expressing and sequencing the hIL-17 gene. By DNA sequencing, the cloned hIL-17 is identical to the reptorted, and with temperature inducing, the hEL-17 was highly expressed in E. coli up to 30% of bacterial protein. The expressed hIL-17 protein could stimulate primary human fibrob-lasts to secrete IL-6 and GM-CSF after initial purification. This results indicate that the expressed hIL-17 has biological activity.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Cancer Biotherapy Year: 1994 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Cancer Biotherapy Year: 1994 Type: Article