Construction of cDNA Library of Ovarian Carcinoma / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)1996.
Article
in Chinese
| WPRIM
| ID: wpr-581892
ABSTRACT
We isolated polyA~+ mRNA direcdy from tumor tissue of ovarian carcinoma using Oligotex~(TM) Direct mRNA Kit (QIAGEN) to synthesize the first and second strand cDNA. The ds-cDNA termini were blunted with pfu DNA poly-rnerase. The blunted cDNAs were added EcoR I adaptor, and then were digested by Xho I . Small cDNA molecules(
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cancer Biotherapy
Year:
1996
Type:
Article
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