The Influence of Polycationic Polypetride on the Transfective Efficacy of NonViral Vector / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)1994.
Article
in Chinese
| WPRIM
| ID: wpr-581935
ABSTRACT
Objective:
Purpose to investigate the different in vitro function of targetable non-viral vector containing poly-L-lysine or protamine.Methods:
Using GV1 and GV2 targetable non-viral vectors, the influences of the poly-L-lysine and protamine on in vitro gene transfer efficiency and the course of gene expression were observed.Results:
?-galactosidase was expressed at intermediate level (50% ) in A375 cells using a complex containing either protamine or poly-L-lysine. Howerver, in case of ABAE cells, ?-galactosidase expression level was low (20% ) transferred with a comPlex containing protamine. On the contrary, ?- galactosidase expression was at high level (70% ) provided that protamine was replaced with poly-L-lysine. In addition, ?-galac- tosidase activity reached the peak at the 6th day after transfection with the complex containing protamine. The expression was not altered with subsequent subcultures, at least for 3 passages. Using poly-L-lysine, the expression peak in A375 reached the peak at the 7th day after transfection, but the level declined along with subsequent passages of cells.Conclusion:
The apllication of protamine in VEGF receptor mediated gene delivery system was limited.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cancer Biotherapy
Year:
1994
Type:
Article
Similar
MEDLINE
...
LILACS
LIS