Molecular composition of Enzymolyzed Anchovy Soluble Peptide and its of Nutrition Evaluation / 中国海洋药物

ABSTRACT

The peptides molecular compositions and the amino acids component of the enzymolyzed protein of anchovy soluble peptides hydrolysed by pepsin and trypsin were assayed and determined by gel HPLC and amino acid analytical instrument.The molecular weight of the peptides is less than 7400, of which 1.74 percent is a large peptide chain with composition of 52~58 amino acids and 6600~7400 molecular weight,29.75 percent is a middle large peptide with 20~41 amino acids and 2500~5300 molecular weight , and 50 percent is a oligo-peptide with 2~10 amino acids and less than 1000 molecular weight. The proportion of total nitrogen to rree amino acid nitrogen is 25.91 in the hydrolysates. 96 pecent of amino acid exists in form of peptides and 4 percent are free amino acids. The con tent of whole amino acids accounts for 73.98 percent soluble peptides, and the essential amino acid is 32.39 percent or 43.78 percent of the whole amino acids .Comparing whth FAO/WHO , the phenylalanine expresses in the first limited amino acid with the amino acid score of 61. Based on the analysis results, the balance of amino acid composition pf anchovy loluble peptides and the physiological function of loigo-peptide in an animal organism were explored.