Expression and Purification of Recombinant Human Endostatin / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)1995.
Article
in Chinese
| WPRIM
| ID: wpr-582543
ABSTRACT
Objective:
To highly express rh-Endostatin from Pichia pastoris and purify it to homogeneity.Methods:
Constructed Pichia pastoris X33/pLW202 was amplified and inoculated to ferment media. The supernatant of the strain was collected after induction. Through ultrafiltration, affinity and gel chromatography, rh-Endostatin with high purication was obtained.Results:
60mg/L rh-Endostatin was obstained from supernatant. HPLC showed its purity was above 98%.Conclusion:
High level expression of secreted rh-Endostatin has been successfully achieved in Pichia pastoris expression system.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cancer Biotherapy
Year:
1995
Type:
Article
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