Determination of Free Thiols in the Chimeric Protein PfCP-2.9 of Plasmodium faldparum / 中国寄生虫学与寄生虫病杂志
Chinese Journal of Parasitology and Parasitic Diseases
;
(6)1987.
Article
in Chinese
| WPRIM
| ID: wpr-582985
ABSTRACT
Objective To determine the free thiols in the chimeric protein PfCP-2. 9 of Plasmodium falciparum expressed by Pichia pastoris. Methods Two experiments of reverse phase HPLC and Ellman' s reaction were applied to the PfCP-2.9 for the determination of its free thiols. For RP-HPLC analysis, three kinds of samples were tested PfCP-2. 9, dithiothreitol-reduced PfCP-2.9 and indoacetic acid-alkylated PfCP-2.9. Results Both experiments showed that there were no any free thiols present in the PfCP-2. 9. Conclution The disulfide bonds between cysteine residues of PfCP-2. 9 were formed completely.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Parasitology and Parasitic Diseases
Year:
1987
Type:
Article
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