Detecting a novel CXC chemokine macrophage inflammatory protein-2? mRNA by real-time fluorescent quantitative reverse transcription polymerase chain reaction (TaqMan) / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-583021
ABSTRACT
Objectives To develop and evaluate a real-time fluorescent quantitative RT-PCR method for the detection of a novel CXC chemokine macrophage inflammatory protein-2?(MIP-2?)mRNA expression based on TaqMan technique.Method The expression of MIP-2? mRNA was detected by TaqMan real time fluorscet quantitative RT-PCR. Results The dynamic range of the assay varied from 102 to 107 copies. The intra- and inter-assay coefficient variation (CV) were less than
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Laboratory Medicine
Year:
2001
Type:
Article
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