The functional proteome preliminary study of rat arterial smooth muscle cells promoted by homocysteine / 医学研究生学报
Journal of Medical Postgraduates
; (12)2003.
Article
in Zh
| WPRIM
| ID: wpr-583691
Responsible library:
WPRO
ABSTRACT
Objective:We investigated the changes of the proteins in the rat arterial smooth muscle cells induced by homocysteine.Detected the mechanism by which homocysteinemia leads to atherosclerosis. Methods:The rat arterial smooth muscle cells of primary culture were planned to be the experimental systems.Extracted the proteins from the smooth muscle cells which were induced by homocysteine and control.After 2-D electrophoresis,the map was analyzed by imaging systems. Results:After cells promoted by homocysteine, twenty-eight proteins of the smooth muscle cells changed.Twelve proteins were found to differ in quantity(11 higher and 1 lower).Sixteen proteins were seen to be unique in one region or the others(12 points of protein appear after Hcy stimulating,4 points of protein disapear after Hcy stimulating). Conclusion:The protein of rat arterial muscle cells changed after promoted by homocysteine.We considered that the proteins were related to the proliferation of the smooth muscle cells.
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WPRIM
Language:
Zh
Journal:
Journal of Medical Postgraduates
Year:
2003
Type:
Article