7?-hydroxycholesterol reduces the extent of reactive gliosis caused by Fe~(3+) in rat brain / 临床神经病学杂志

ABSTRACT

Objective To demonstrate anti-proliferative property and significance of 7?-hydroxycholesterol(7?OHCH)on astrocytes after brain injury.Methods The rats were given a cortical injection of ferric chloride, then immediately infused liposome suspension with or without 7?OHCH in the injury site. Glial fibrillary acidic protein(GFAP) expression in cortex detected quantitatively with immunohistochemistry and computerized image analysis.Results A mass of inflammatory cells infiltrated and the number of GFAP positive astrocytes increased greatly around the injury site in rats with injection of iron,whereas the structure of cortex and the number of GFAP positive astrocytes around the injury site in the rats with injection of iron and 7?OHCH were as same as normal control.Conclusion 7?OHCH has anti-proliferative property on astrocytes, and this will provide a new way to prevent reactive gliosis and facilitate brain functional recovery following brain injury.