The role of signal transduction molecule PLC in streptococcus pneumoniae inducing filamentous actin rearrangement in type n pneumocytes in vitro / 中华检验医学杂志

ABSTRACT

Objective To investigate whether S. pn can provoke filamentous actin (F-actin) rearrangements in vitro , which will further lead to S. pn invasion of A549 and the relationship between PLC signaling molecule and. the invasion events. Methods Labelled F-actin with FITC-phalloidin, we observed F-actin rearrangements by S. pn adhesion of type Ⅱ pneumocytes ( A549). S. pn invasion of A549 cells was determined by pretreating A549 cells with Cytochalasin D . To investigate whether F-actin rearrangements can be blocked by PLC inhibitor, A549 cells were pretreated with PLC inhibitors U73122. Results Intact S. pn can promote F-actin rearrangements. Cytochalasin D is able to prevent S. pn invasion of A549 cells. Inhibitors of PLC signal transduction molecules block F-actin rearrangements dose dependently. Conclusion S. pn can provoke F-actin rearrangements through PLC signaling pathways, which will further lead to S. pn invasion of A549 cells.