Factors influencing the assay of ?_1-antitrypsin bioactivity / 中国输血杂志

ABSTRACT

Objective To study the factors influencing the assay of ? 1 antitrypsin(? 1 AT) bioactivity. Methods Various concentrations of PEG(MW, 4000), sucrose, sodium citrate and ethanol were added before determining ? 1 AT activity. Results ? 1 AT activity increased approximately 100% compared with the controls when the PEG concentration reached 20%. Conclusion PEG concentration influences the ? 1 AT activity assay.