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Anti-Tumor Efficacy Induced by Antibodized PDTRP Gene Immunization / 中国肿瘤生物治疗杂志
Article in Chinese | WPRIM | ID: wpr-584112
Responsible library: WPRO
ABSTRACT

Objective:

To study the anti-tumor efficacy induced by antibodized tumor epitope PDTRP gene immunization.

Methods:

Three copies of tumor associate gene PDTRP from MUCI tandem repeats were designed and mimicked the conformation of MUCI by Insight Ⅱ . The ?lneo-PDTRP plasmid was further constructed, in which the PDTRP target gene was inserted into CDR3 of the ?1 -neo vector. The specific humoral and cellular immune responses towards to PDTRP were detected after intraspleen immunized Balb/c mice with "ylneo-PDTRP. And the immune protection assay was also done to observe whether the mice immunized with ?lneo-PDTRP could prolong the survival after tumor challenge.

Results:

The conformation of three copies of PDTRP mimicked the conformation of MUCI tandem repeats. The expression of ?lneo-PDTRP could be detected after in vitro transfect. The specific antibody against PDTRP epitope could be induced and increase to a higher titer after intraspleen injection with a ?lneo-PDTRP plasmid. And the specific proliferation and cytotox-ic function of lymphocyte were also increased. There is a significant survival from mice immunized with ?lneo-PDTRP a-gainst the 4T1-PDTRP tumor challenge.

Conclusions:

Gene immunization with ?lneo-PDTRP could elicit both humoral and cellular tumor specific immune response and had the protective effect.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Cancer Biotherapy Year: 1995 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Cancer Biotherapy Year: 1995 Type: Article