The extraction and identification of lipid rafts / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2004.
Article
in Chinese
| WPRIM
| ID: wpr-584514
ABSTRACT
Objective:
To establish a method using non-ionic detergent for extracting lipid rafts.Methods:
Because lipid rafts can resist solubilization by non-ionic detergents under 4℃, we use non-ionic detergent (Triton X-100) to treat with epicyte fractions, the non-raft membrane would be solubilized. Then we utilize sucrose gradient centrifugation, preparations enriched in lipid rafts could be obtained.caveolin-1 was used as markers of lipid-raft structures.Results:
A white light-scattering band under light illumination located at the interface between 15%-20% sucrose was detectable, and a brown stripe which comparative molecular quantity is 24 000 was identified by Western-Blot analysis.Conclusion:
The method using non-ionic detergent is simple and useful for extracting lipid rafts, extracting lipid rafts would be prerequisite in studying the function of lipid rafts.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Journal of Medical Postgraduates
Year:
2004
Type:
Article
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