Laboratory diagnosis of leukocyte myeloperoxidase deficiency / 中华检验医学杂志

ABSTRACT

Objective To study the pathological characteristics of leukocyte myeloperoxidase (MPO) deficiency and clinical laboratory diagnostic strategy for this disease. Methods The Bayer ADVIA 120 blood cell analyzer differentiate leukocyte and detect myeloperoxidase index (MPXI) . The leukocyte morphology and differentiating count on blood smear by Wright’s stain were finished. The peroxidase positive cell percentage and score were counted by peroxidase stain. The expressive levels of leukocyte MPO antigen were measured by flow cytometric monoclonal antibodies method.Results The prevalence of mild neutrophil MPO deficiencies was 2.61%, and partial neutrophil MPO deficiencies was 0.39% in 5 761 in-patients. The significant changes in the dot plot from ADVIA 120 blood cell analyzer were seen in 3 patients with MPO deficiencies, and MPXI,MPO activities and MPO antigen levels of the patient’s neutrophils and monocytes decteased remarkably,but there were normal levels in eosinophils.Conclusion Neutrophil MPO deficiencies were not a rare disorder,but the mild deficiency cases were seen usually.The low activity of MPO is an important evidence for MPO deficiencies diagnosis.The Bayer ADVIA 120 blood cell analyzer can be used simply and fleetly for screening MPO deficiencies in routine hematology laboratory.The peroxidase stain of blood smear is an improtant diagnostic method for MPO deficiencies.