Clinical study on effect of common methylenetetrahydrofolate reductase gene mutation on coronary artery disease in hypertension / 中国介入心脏病学杂志

ABSTRACT

Objective To investigate the association between the MTHFR gene C677T mutation and CAD in patients with hypertension. Methods Of the 103 cases of patients with hypertension who underwent coronary angiography, 58 were CAD and 45 were not. MTHFR genotypes were identified by PCR and restriction fragment length polymorphism analysis with Hinf I digestion. Plasma folate was determined by radioimmunoassay. Plasma HCY was determined by HPLC. Results The frequencies of T677 allele (0.45) in the CAD group were markedly higher than those in the control group (0.33, P=0.03), plasma homocysteine level in the CAD group (17.73?2.22 ?mol/L) was also significantly higher than that in the control group (12.18?1.23 ?mol/L, P=0.037). There was no significant difference in the concentrations of folate for the two studied groups. Conclusion The MTHFR gene T677 variant is the genetic high risk factor for CAD in patients with hypertension. It can induce the hyperhomocysteinemia, potentially contributing to the pathogeny of CAD.