Expression and identify of CH50 polypeptide in bladder cancer cell line BIU-87 / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-584692
ABSTRACT
Objective:
To investigate the expression and identification of CH50 polypeptide in bladder cancer cell line BIU-87 by gene transfection.Methods:
The eukaryotic expressing vector pCH510 of polypeptide CH50 was introduced into BIU-87 cells by gene transfection Lipofectimine TM2000.The expressed product was identified by immunohistochemistry and Western blot method. The expression of the transfected gene was identified by RT-PCR.Results:
The BIU-87 cells could produce CH50 polypeptide by transfection.Conclusion:
When the vector of pCH510 was transfected into BIU-87 cells in vitro, the cell adhension characteristic would be changed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Journal of Medical Postgraduates
Year:
2003
Type:
Article
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