Synthesis of lactosaminated carboxymethylchitosan and its action of plasmid DNA delivery / 中国海洋药物
Chinese Journal of Marine Drugs
;
(6)1994.
Article
in Chinese
| WPRIM
| ID: wpr-584979
ABSTRACT
Objective To study the synthesis of lactosaminated carboxymethylchitosan (Lac-CMCS) and its action of delivering plasmid DNA into cells. Methods The lactosaminated carboxymethylchitosan was synthesized by reductive amination and was conjugated with plasmid contained antisense x gene. This complex was delivered into cells and these cells were cultured in 37℃,5%CO_(2). Testing the inhibition on HBV by determining HBsAg and HBeAg produced by HepG2.2.15 cells with ELISA technique. Results The molar ratio between lactose and CMCS was 15∶1 in this covalence conjugate. The best mass ratio of DNA to Lac-CMCS was 1∶10 in DNA/Lac-CMCS which was able to deliver gene into cells, and the ma-ximal inhibition rate was 83.6%. Conclusion In vitro, the lactosaminated carboxymethylchitosan can effectively deliver DNA into cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Marine Drugs
Year:
1994
Type:
Article
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