A convenient and fast method to amplify DNA fragments from filamentous fungi / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-585000
ABSTRACT
Objective:
Using PCR to amplify filamentous fungi DNA fragments.Methods:
Pour some liquid nitrogen to the hypha and grind them to powder. Then add some TE buffer and boil them for 15-20 minutes. Take a little supernatant as PCR template. After PCR do agarose gel electrophoresis. Collect the gel containing the objective fragment and add some TE. Take a little supernatant as PCR template (again.) After PCR, do agorose gel electrophoresis again.Results:
We obtained a great deal of objective DNA.Conclusion:
Amplifying filamentous fungi DNA fragment by this way, we can save time and money. We can also improve our work efficiently.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Medical Postgraduates
Year:
2003
Type:
Article
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