Effect of rh-GH on myocardial angiogenesis of rats with acute myocardial infarction / 中国介入心脏病学杂志
Chinese Journal of Interventional Cardiology
; (4)1996.
Article
in Zh
| WPRIM
| ID: wpr-585123
Responsible library:
WPRO
ABSTRACT
Objective To determine the effect of intramuscular administration of rh-GH on myocardial angiogenesis and the expression of bFGF, VEGF in rats with acute myocardial infarction. Methods Left anterior descending coronary arteries have been ligated in 50 Wistar rats after anesthesia by 10% pentobarbital sodium. 37 rats with AMI survived after the operation. The rats are randomized into rh-GH treatment group (n=19) and the control group (n=18). Treatment group is given rh-GH 0.25 U/kg?d im for three weeks while the control group is given NS im at the same time. All the 37 rats are killed after 3 weeks and the heart specimen were fixed by injecting 10% formalin from the ascending aorta into the coronary arteries. The level of VEGF and bFGF in blood plasma and myocardium as well as the density of newly formed vessels in the peri-infactional area were measured separately. Plasma VEGF and bFGF were measured before experiment and 3 weeks after experiment by enzyme-label method.bFGF and VEGF in myocardiun were semi-quaot fixed by imaging after SP inmuno-histochemical stain. Left ventricle capillary density 3 angiogenesis sites was identified by 40? or 100? microscopy. F8 labelled microvessels were measured averagely under 200? microscope. Results (1) Plasma VEGF and bFGF in both groups were elevated, and elevation of the treatment group was more remarkable than the control group 69?5 vs 36?4 (P
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1
Index:
WPRIM
Type of study:
Clinical_trials
Language:
Zh
Journal:
Chinese Journal of Interventional Cardiology
Year:
1996
Type:
Article