The Production Influencing Factors of Extracellular Polysaccharide(EPS) from a Strain of Lactic Acid Bacteria and EPS Extraction / 微生物学通报

ABSTRACT

The influencing factors of EPS produced from LAB were studied by using the Phenol-H_(2)SO_(4 )method. It was demonstrated that the strain produced EPS at the most amount when it was incubated to 40~48h and pH4 under 30℃. Glucose is the most suitable sugar source for LAB producing EPS. The rough EPS was obtained from LAB' culture after centrifugation, dialysis, deprotein, decoloration, and ethanol-precipitation. The sample was at least composed of two polysaccharides that were different completely from the molecule weight and the amount. The purified EPS was got through the SephadexG-200 column and it was showed that it was a purified sample by thin layer chromatograghy.