Application of Autoradiography in Study of Cold Storaged Transplant Organs / 中国医科大学学报
Journal of China Medical University
;
(12): 101-102,107, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-585332
ABSTRACT
Objective:
Our purpose was to study whether exogenous adenosine triphosphate (ATP) could enter liver cell or not.Methods:
Using continuous hypothermic machine perfusion model of rat liver, we used autoradiography in this experiment.Results:
The light microscope pictures of group A(no [α-32P] ATP in the perfusate) showed that there was no specially labeled silver pellet in or out of rat liver cell. But the light microscope pictures of group B (37 MBq [α- 32P]ATP in the perfusate) showed that distribution of numerous [α-32P]ATP autoradiographic silver pellets in rat liver cell, and no autoradiographic silver pellet was found in the hepatic sinus and vessel.Conclusion:
Exogenous ATP could enter cold storaged rat liver cell.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of China Medical University
Year:
2001
Type:
Article
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