Effect of Sargassum hemiphyllum polysaccharides on the mRNA expression of CD_(34)~+ cells and SCF in irradiated mice / 中国海洋药物

ABSTRACT

The mice were administered with 10~40mg?kg~(-1)?d~(-1) SHP [Sargassum hemiphyllum(Turner) C.Agardh polysaccharides,SHP] ip for 7d before 5Gy ?-rays irradiation to observe the effect of SHP on the mRNA expression of CD~+_(34) and SCF(stem cell factor),and to study the mechanism of SHP against irradiation on the hematopoietic system in irradiated mice.Flow cytometry was used to detect number of bone marrow cells and the mRNA expression of CD~+_(34) and SCF were analysed by RT-PCR test.The results showed that the percentage of CD~+_(34) cells was decreased by ?-rays irradiation(5Gy) and SHP(10～40mg?kg~(-1)) could increase significantly the percentage of CD~+_(34) cells to exhibit a protective effect of SHP on bone marrow in irradiated mice.Expressions of CD~+_(34) and SCF mRNA were lower in model group than those in conrol group and the expressions were higher in three test groups than those in model group.The results indicated that the increases of expressions of CD~+_(34) and SCF mRNA could be a mechanism of SHP against irradiation on the hematopoietic system in mice.