Immunohistochemical Study for MAPK Expression in UV Irradiated Melanocytic Nevi / 대한피부과학회지
Korean Journal of Dermatology
;
: 1186-1191, 2005.
Article
in Korean
| WPRIM
| ID: wpr-58554
ABSTRACT
BACKGROUND:
In contrast to the relationship between UV exposure and nonmelanoma skin cancers, molecular evidence for the involvement of UV radiation in melanocytic tumorigenesis is quite limited. Mitogen-activated protein kinase (MAPK) is an important molecule in transducing extracellular signals from the cell surface to the nucleus. The MAPK family includes ERK (extracellular signal- regulated protein kinase), JNK (stress-activated c-Jun N-terminal kinase), and p38 kinases. Various growth factors and cytokines, as well as other signals such as UV light are able to activate MAPK, resulting in a variety of cellular responses including proliferation, differentiation and apoptosis.OBJECTIVE:
The purpose of our study was to investigate the effect of UV irradiation on the expression of MAPK in melanocytic nevi.METHOD:
Melanocytic nevi from ten healthy volunteers were partially covered, irradiated with a defined UV dose, and excised after 1 week. healthy The irradiated and non-irradiated area were examined separateted by immunohistochemistry using p-ERK, p-JNK, and p-p38 monoclonal antibodies.RESULTS:
In the non-irradiated area of melanocytic nevus, p-ERK, p-JNK, and p-p38 were undetectable in nevus cells. After irradiation, p-ERK expression was observed in nevus cells in 7 cases, and p-p38 was stained diffusely in the cytoplasm of nevus cells in 6 cases, but there was no immunoreactivity of p-JNK.CONCLUSION:
We suggest that a single UV irradiation of melanocytic nevi can stimulate both p-ERK and cytoplasmic p-p38 expression, but not p-JNK.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphotransferases
/
Protein Kinases
/
Skin Neoplasms
/
Ultraviolet Rays
/
Immunohistochemistry
/
Cytokines
/
Apoptosis
/
Cytoplasm
/
Intercellular Signaling Peptides and Proteins
/
Carcinogenesis
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Dermatology
Year:
2005
Type:
Article
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