Effects of GSRb3 on GABA in brain tissue of rats with injury of hypoxia / 临床神经病学杂志

ABSTRACT

Objective To observe the influence of ginsenoside Rb3(GSRb3) on ?-aminobutyric acid (GABA) in brain tissue of rats with injury of hypoxia.Methods 20 rats were randomly divided into A, B, C, D and E group. The models of cerebral anoxia in group A and C were made by hypopiesia and hypoxia, and the models of hypoxia-reoxgenation were made in group B and D. 24 h and 1 h before establishment of models, ginsenoside Rb3 was injected peritoneally in rats of group C and D, respectively. The number and form of GABA-like immunoreactive neurons in hippocampus CA1 area of rats after brain hypoxia-reoxgenation were investigated with immunohistochemistry.Results (1)Compared with group E, GABA-like immunoreactive neuron density in CA1 area of group A and B decreased, presented with light staining and absence of prominency. GABA-like immunoreactive neuron density in CA1 area in group C and D was significantly higher than that in group A and B, but the shape was similar to group E. (2) The number of GABA-like immunoreactive neuron in CA1 area was 7.7?2.83 (group A), 10.1?2.08 (group B), 30.9?2.02 (group C), 33.1?4.2 (group D) and 16.9?1.05 (group E), respectively. The numbers were lower in group A and B than in group E, however higher in group C and D than in group E(all P