Cloning and Sequence Analysis of Aniline Dioxygenase Gene / 微生物学通报

ABSTRACT

With specific primers designed,the aimed DNA fragment was amplified by PCR from aniline-degrading strain ANA5.A genomic library of strain ANA5 was constructed in the cosmid vector pLAFR3 using E.coli EPI100 as the host strain.Two recombinants were identified from the genomic library using in situ hybridization and Southern blotting probed with the PCR product.By the analysis of subclone sequencing,it was sure that the aniline dioxygenase gene of strain ANA5 was cloned.The sequence analysis and the deduced amino acid showed that they were different from the relative sequences registered in the GenBank and revealed that aniline dioxygenase gene was conserved through evolution.