Inhibitory effects of polypeptides from Chlamys farreri(PCF) on HaCaT cells apoptosis induced by UVB radiation / 中国海洋药物

ABSTRACT

Objective To investigate whether PCF could inhibit apoptosis of HaCat cells induced by ultraviolet B(UVB) in vitro and explore its related mechanism.Methods HaCat cells were cultured and divided into groups(control,model,and test groups).The DNA ladder of each group was observed by gel electrophoresis;The expression of caspase-3 in HaCat cells was detected by flow cytometry and the activities of ERKs,JNKs,P38 were determined by western-blot method.Results PCF decresded UVB-induced DNA ladder in HaCat cells;PCF enhanced the level of ERKs,but reduced the level of JNKs and p38,and reduced the expression of Caspase-3 in HaCat cells.Conclusion PCF had protective effects against UVB-induced apoptotic cell death in HaCat cells and it is likely that its cytoprotective effect on HaCat cells was mediated by MAPKs pathway.