Effect of oxidized low density lipoprotein on CD36 expression in macrophage / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2004.
Article
in Chinese
| WPRIM
| ID: wpr-585758
ABSTRACT
Objective:
To investigate the effect of oxLDL on CD36 expression and cholesterol influx in THP-1 macrophage.Methods:
THP1 macrophage were treated with 50mg/L oxLDL for 0,12,24,36,48 h respectively.Oil red O staining was used to observe the intracellular lipid droplets.\labeled Cholesterol influx was determined by FJ-2107P type liquid scintillator.CD36 mRNA and protein level were determined by reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting respectively.Results:
We found that oxLDL elevated CD36 in both protein and mRNA levels and increased cholesterol influx in a time-dependent manner.The levels of cholesterol influx were 23.5%、(27.8%、)39.7%、44.1% and 49.7% respectively.Conclusion:
oxLDL may upregulate CD36 expression and increase cholesterol influx.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Medical Postgraduates
Year:
2004
Type:
Article
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