Tet Regulating Expression System Establishment and Functional Analysis ofNovel Gene STGC3 in Nasopharyngeal Carcionma Cell Line CNE2 / 生物化学与生物物理进展

ABSTRACT

In an attampt to establish the functional expression of STGC3 with doxycycline (Dox) induced Tet-onregulating system in human nasopharyngeal carcinoma cell line CNE2, an ideal experimental platform wasprovided for further studies of STGC3. pTet-on regulating plasmid was transfected into CNE2, and stableexpression of Tet-on was established in CNE2 through G418 select. Then the response plasmid of recombinantpTRE-STGC3 was steadily transfected into positive CNE2/Tet-on cells with hygromycin screen. Dox was used toinduce the expression of STGC3 and a cell clone sensitive to Dox was selected. The best-induced concentrationwas determined with different concentration of Dox induction. Growth curves, clone formation rate and cell cycledistribution were detected after STGC3 gene up-regulated expression with Dox induction. The growth capacity andclone formation potential of CNE2/Tet /pTRE-STGC3 was significantly suppressed, compared with the controls(P