Expressions of Bcl-2 and Bax in cerebral ischemic preconditioning-perfusion models of rats / 临床神经病学杂志

ABSTRACT

Objective To observe the expressions of apoptosis-related proteins Bcl-2 and Bax in cerebral ischemic preconditioning-perfusion models of rats. Methods Ischemic preconditioning was induced by 20 minutes of monolateral middle cerebral artery occlusion using an intraluminal filament method in rats. The animals were perfused 6 h, 12 h, 1d, 3 d, 5 d and 7 d respectively before they were sacrificed. The expressions of Bcl-2 and Bax were analyzed by immunohistochemistry staining. Results Bcl-2 positive cells increased significantly at 1 d after perfusion, reached their peak at 3 d, and maintained high level until 7 d. They were all higher than that of control group (all P 0.05). Conclusion Upregulation of Bcl-2 but not Bax may play a role in ischemic preconditioning protection .