The expression and effect of IL-18 after hypoxic-ischemic brain damage / 中华检验医学杂志

ABSTRACT

Objective To explore the expression and effect of interleukin(IL)-18 after hypoxic-ischemic brain damage(HIBD) in neonatal rats. Methods The HIBD model of seven-day-old Wistar rats was established. The mRNA and protein for IL-18 in cerebral cortex of control group, HIBD3 h, 8 h, 24 h, 3 d, 6 d and 14 d group were analyzed by RT-PCR and immunohistochemistry respectively, at the same time histological changes were observed. Results The expression of IL-18 mRNA and protein was low in control group[mRNA 0.321 8?0.046 6; protein (6.033? 1.019)cells/field]. After HIBD, the level of mRNA/protein for IL-18 in ischemic cortex increased progressively at 24 h to 6 d [mRNA 24 h 0.582 3?0.074 0, 3 d0.697 6 ?0.107 3, 6 d 0.911 0?0.064 7; protein 24 h (19.133?2.094)cells/field, 3 d (28.900 ?1.589) cells/field, 6 d (52.883?3.203)cells/field; P