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Studies on The Interactions Between NIRF and P53 / 生物化学与生物物理进展
Progress in Biochemistry and Biophysics ; (12)2006.
Article in Chinese | WPRIM | ID: wpr-586051
ABSTRACT
HEK293 or HeLa cells were transfected by NIRF and, or P53, whole cell extracts andimmunoprecipitates were subjected to SDS-PAGE followed by Western blotting. GST pull-down was carried outto identify the interactions between NIRF and P53. In vitro ubiquitination reaction was carried out to identify P53ubquitinate by NIRF. The results suggested that NIRF could interact with P53 in vivo and in vitro. The results alsoshowed that NIRF could ubiquitinate P53 in vivo and in vitro. The results indicated that NIRF would be a newnegative regulator of P53.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Progress in Biochemistry and Biophysics Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Progress in Biochemistry and Biophysics Year: 2006 Type: Article