Renoprotective effect of reduced glutathione in diabetic rats and its mechanism / 吉林大学学报(医学版)

ABSTRACT

Objective To investigate the renoprotective effect of reduced glutathione on streptozotocin-induced diabetic rats and its possible mechanism.Methods Streptozotocin-induced diabetic rats received aminoguanidine(50 mg?kg~(-1)?d~(-1)) and reduced glutathione 400 mg?kg~(-1)?d~(-1) intraperitoneally respectively or synchronously for(8 weeks.) The expression of TGF-?1 mRNA and protein in renal cortex were determined by reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemistry respectively.The mean glomerular area(MGA) and volume(MGV) were measured by image analysis system.The changes of creatinine clearance rate(Ccr),the kidney weight/body weight ratio and the urinary albumin excretion rate(UAER) were determined.Results By the end of 8 weeks,the Ccr,UAER,MGA,MGV,kidney weight/body weight ratio,the contents of TGF-?1 mRNA in renal cortex were increased significantly in DM groups compared with the blank control group(P