Effect of rmu-IFN-? on the Splenic T Cell Subsets and NK Cells of Pregnant Mice Infected with Toxoplasma gondii / 中国寄生虫学与寄生虫病杂志

ABSTRACT

Objective To explore the effect of rmu-IFN-? on the change of T cell subsets and natural killer cells of pregnant mice infected with T.gondii.Methods Early pregnant mice infected with T.gondii were administered with different doses(1 U/g or 10 U/g)of rmu-IFN-? for three days before euthanasia.The numbers of splenic CD4+ and CD8+ T cells and natural killer cells were detected by flow cytometry.Results Compared with the infected mice that were not treated,the level of splenic CD4+ T cells in mice administered with two doses of rmu-IFN-? increased on the day 10,12,14 of gestation,while the level of CD8+ T cells decreased on the day 10,14 of gestation.The ratio of CD4+/CD8+ T cells increased significantly on the day 10,12,14 of gestation.Survival days of the two administered groups were longer than those of the infected group.Conclusion A proper dose of rmu-IFN-? can reverse the decline of the ratio of T cell subsets,improve the proliferation of NK cells,and so increase the level of peripheral cellular immunity of pregnant mice.