The Effect of alpha-MSH on Long-term Histologic Changes after Acute Ischemic Kidney Injury in Rats / 대한신장학회잡지
Korean Journal of Nephrology
;
: 191-203, 2005.
Article
in Korean
| WPRIM
| ID: wpr-58658
ABSTRACT
BACKGROUND:
Ischemic acute renal failure (ARF) is increasingly recognized as involving chronic functional and structural sequelae. Ischemia reperfusion injury (I/R) plays a major role in delayed graft function and long-term changes after kidney transplantation, also. The present study was designed to evaluate the long-term changes after acute ischemic injury and whether renoprotection by alpha-MSH in the acute ischemic stage, could reduce the long-term sequelae.METHODS:
In control group, ischemia/reperfusion injury was induced in male Sprague-Dawley rats by clamping Lt. renal pedicle for 15, 45, 60 minutes after removal of Rt. Kidney and followed by reperfusion. The animals in alpha-MSH group were injected alpha-MSH prior to reperfusion and then every day for 1 week. We measured BUN, creatinine at 24 hour after I/R injury, and in each group, 6 animals were sacrified at 1 week and 4 weeks after I/R injury to evaluate apoptosis, ED1, PCNA, and histopathologic changes.RESULTS:
At 4 weeks after I/R injury, the remnant structural damage such as apoptosis, ED1 stained cells, MT (+) tubulointerstitial fibrosis were observed. alpha-MSH could reduce the initial functional injury, and apoptosis, ED1 stained monocyte, MT (+) tubulointerstitial fibrosis, also.CONCLUSION:
Renal function was recoverd at 4 weeks after I/R injury, but structural sequelae such as apoptosis, fibrosis were remnant. alpha-MSH could attenuate initial functional damage and remnant fibrosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Fibrosis
/
Alpha-MSH
/
Monocytes
/
Reperfusion
/
Reperfusion Injury
/
Kidney Transplantation
/
Rats, Sprague-Dawley
/
Apoptosis
/
Proliferating Cell Nuclear Antigen
/
Constriction
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
Korean Journal of Nephrology
Year:
2005
Type:
Article
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