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Identification and characterization of the integrated site of pseudomonas aeruginosa phage PaP3 / 医学研究生学报
Article in Zh | WPRIM | ID: wpr-586589
Responsible library: WPRO
ABSTRACT
Objective: Identification of the attachment site of phage PaP3 within the genome of Pseudo-monas aeruginosa PAS. Methods:The full genome of lysogenic bacteria was cleaved by Pst Ⅰ and produce a large fragment of more than 45 000 bp, which was subsequently digested by EcoR Ⅰ. Then the fragment containing DNA sequence of phage and bacteria was cloned into pFastBacTMHT A vector, and the result of sequencing indicated the right hybrid site attR. AttL was isolated by PCR on the base of integration mechanism. And then attP and attB were indentified according to the nucleotide sequences of attR and attB. Results:A sequence of 21 bp(5'-GGTCGTAGGTTCGAATCCTAC-3') was defined to be the core site of integration, which was located at t-RNAPro gene in the genome of phage PaP3 and t-RNALys gene in the genome of Pseudomonas aeruginosa PA3. The attP and attB flanked with a set of inverted repeat and direct repeat. Conclusion:The integrated site of PaP3 within the genome of PA3 was identified and characteriged, which could be of value in investigating the mechanism of integration and gene flow between different species in the natural world.
Key words
Full text: 1 Index: WPRIM Type of study: Diagnostic_studies Language: Zh Journal: Journal of Medical Postgraduates Year: 2003 Type: Article
Full text: 1 Index: WPRIM Type of study: Diagnostic_studies Language: Zh Journal: Journal of Medical Postgraduates Year: 2003 Type: Article