Protective effects of antipsychotic drugs Venlafaxine and Olanzapine on pheochromocytoma(PC12) cells in serum-free medium / 吉林大学学报(医学版)

ABSTRACT

Objective To study the protective effects of antipsychotic drugs Venlafaxine and Olanzapine on pheochromocytoma(PC12) cells after serum withdrawal.Methods The cells were cultivated in serum-free medium.Venlafaxine and Olanzapine were added in the medium.Morphological observation,MTT assays and LDH assays were used to study the protective effects of Venlafaxine and Olanzapine.Results Venlafaxine and Olanzapine prevented PC12 cells from the damage in serum-free medium,elevated PC12 cell survival rates,reduced the LDH release and kept cell membrane intact.The greatest effects were observed at concentrations of 100 ?mol?L~(-1) Olanzapine and 20 ?mol?L~(-1) Venlafaxine(P