Expression of IFN-? and IL-12 in peripheral blood mononuclear cells of patients with chronic hepatitis B and its clinical significance / 临床检验杂志

ABSTRACT

Objective To detect the expression of interferon-?(IFN-?) and interleukin 12(IL-12) in peripheral blood mononuclear cells(PBMCs) of patients with chronic hepatitis B and analyze cellular immune status of the patients with chronic hepatitis B virus(HBV) infection.Methods The PBMCs of patients with chronic HBV infection were cultured,then the antiviral biological activity of PBMCs stimulated with high concentration of PolyIC in the culture supernatants was detected by viral protection assay.IL-12 mRNA in PBMCs from the patients with chronic hepatitis B was measured by reverse transcription polymerase chain reaction(RT-PCR).Results The obtained data indicated that lower titer of IFN-?from PolyIC-induced PBMCs existed in patients infected by chronic HBV.Enhanced IFN-?activity from PBMCs stimulated by PolyIC was found in the responder(n=4) who were treated with IFN-?2b.However,continuously lower activity of IFN-?from PBMCs presented in non-responder(n=12) during IFN-?2b therapy.The expressions of IL-12 mRNA in PBMCs from both responder and non-responder were significantly higher than those of normal control group(n=10) before treatment with IFN-?2b(P