Identification of BAC clones for array CGH in human cancer by FISH technique / 吉林大学学报(医学版)

ABSTRACT

Objective To evaluate the quality of BAC clones for CGH microarrays in the detection of tumors.(Methods Chromosome) preparations were made from peripheral blood of the healthy volunteers in the conventional manner.The locations of BAC(RPCI-11 library and CTC library) within the region of interest were compiled from information archived by the UCSC and the NCBI.Probes were labeled by nick-translation with biotin-16-dUTP or digoxigenin-11-dUTP.Precise localization of each BAC was confirmed using normal metaphase chromosomes by FISH technique.The copy number and molecular organization of the region of 223 BAC clones which were crucial in the development and progression of human cancers were investigated.Results The FISH analysis indicated the normal BAC clones accounted for 81.62% of the total(186/223);the abnormal clones with additional FISH noises accounted for 13.45%(30/223);those with wrong localization pattern was 3.58%(8/223),and those with no bacterial growth was 1.35%(3/223),respectively.Conclusion FISH technique is effective and useful in the identification of BAC clones for array CGH.