Effects of Fenofibrate on neonatal rat hypertrophic cardiomyocyte in vitro / 医学研究生学报
Journal of Medical Postgraduates
; (12)2003.
Article
in Zh
| WPRIM
| ID: wpr-587194
Responsible library:
WPRO
ABSTRACT
Objective:To study the inhibitory effects of peroxisome proliferator-activated receptor ? activator Fenofibrate on the angiotensin Ⅱ(Ang Ⅱ)-induced cardiac hypertrophy in vitro. Methods: A model of hypertrophy of neonatal rat cardiac myocytes was established with Ang Ⅱ stimulation.With the aid of Leca Qwin Image software,the surface areas of cardiac myocytes were analyzed.The mRNA expression of ?、?-myosin heavy chains(?-MHC、?-MHC) and PPAR? was measured by reverse transcription-polymerase chain reaction(RT-PCR),and the cultured myocyte viability was estimated by MTT assay. Results:Fenofibrate pretreatment 24 h prior to Ang ⅡSignificantly reduced Ang Ⅱ-induced cardiac hypertrophy,inhibited the effect of AngⅡ on the cardiac myocyte viability and increased expression of?/?-MHC mRNA and PPAR? mRNA in a dose-dependent manner.In contrast,Fenofibrate had no significant effect on Ang Ⅱ treated cardiac myocytes when Fenofibrate treatment was concomitant with Ang Ⅱ. Conclusion: PPAR?-dependent pathway was involved in the inhibition of cardiac hypertrophy,but chronic treatment was needed.
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WPRIM
Language:
Zh
Journal:
Journal of Medical Postgraduates
Year:
2003
Type:
Article