Separation of HSP70-peptide compound and the expression of HSP70 in esophagus cancer tissue / 基础医学与临床
Basic & Clinical Medicine
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-587670
ABSTRACT
Objective The aim is to expore the expression of HSP70 in human esophagus cancer and introduce method of separation of HSP70-peptide compound.Methods The expression of human HSP70 RNA in human(esophagus) cancer was hybridized with P17 probe by Northern blot.The HSP70-peptide complex was isolated by(affinity) chromatography.Results HSP70 mRNA was detected in human esophagus cancer tissue at much more higher level than corresponding normal tissue;HSP70-peptide compound are separated from esophagus cancer of human body.Conclusion This research not only testified the strong expression of HSP70 in esophagus cancer,but also supplied a practical method of extraction and purification of HSP70-peptide compound of tumor origin.This study makes a starting point for further research in this field.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Basic & Clinical Medicine
Year:
2006
Type:
Article
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