Immobilization Method on Piezoelectric Quartz Crystal DNA Sensors Based on Monolayer SAM / 中华医院感染学杂志
Chinese Journal of Nosocomiology
;
(24)2005.
Article
in Chinese
| WPRIM
| ID: wpr-587757
ABSTRACT
OBJECTIVE To discuss a highly effective method to immobilize probe on the surfaces of piezoelectric DNA sensors.METHODS Pseudomonas aeruginosa probe was immobilized on the gold surface of gene sensor(array) with routine self-assembly method(SAM)(non-reduction method) and SAM with deoxidized probe((reduction) method),respectively.The changes in frequency and time-cost were compared in reactions with(different) concentrations of probe.RESULTS Reduction method had the advantage of more probe immobilization;less time consumed in testing and higher changes in frequency during the reaction than non-reduction method.CONCLUSIONS Reduction method has a better ability to immobilize probe on the surfaces of piezoelectric DNA sensors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Nosocomiology
Year:
2005
Type:
Article
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