Effect of telomerase-antisense DNA on apoptosis of hepatoma cells induced by arsenic trioxide / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)2006.
Article
in Chinese
| WPRIM
| ID: wpr-588073
ABSTRACT
Objective:
To observe the effect of telomerase-antisense DNA on apoptosis of hepatoma cells induced by arsenic trioxide, in an effort to look for a new anti-hepatic cancer agent with high efficiency, low cytotoxicity.Methods:
We designed and synthesized a 20nt telomerase-antisense DNA targeting telomerase template and observed its influence on the telomerase activity of hepatoma cells. H-E staining, flow cytometry, and DNA agarose electrophoresis were used to study the preventive effect of telomerase-antisense DNA on hepatoma cells apoptosis induced by arsenic trioxide. Flow cytometry was used to detect the expression of Fas, Fas-L, and bcl-2.Results:
Telomerase-antisense DNA (5 ?mol/L) effectively inhibited the telomerase activity of hepatoma cells after 24 hours (P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cancer Biotherapy
Year:
2006
Type:
Article
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