The Establishment of ELISA Method for Detecting Babesia gibsoni by Recombinant Antigen BgAMA-1 / 中国寄生虫学与寄生虫病杂志
Article
in Zh
| WPRIM
| ID: wpr-588079
Responsible library:
WPRO
ABSTRACT
In order to improve diagnostic method to Babesia gibsoni infection,gene recombination technique was used in our study.By this technique,we constructed and expressed recombinant apical membrane antigen-1(BgAMA-1),and recombinant BgAMA-1 was used in ELISA experiment as diagnostic antigen.The results showed the recombinant expressed plasmid was triumphantly constructed and the recombinant antigen BgAMA-1 had a high specificity in ELISA test.These results demonstrate that the recombinant BgAMA-1 might be a useful diagnostic reagent for detection of antibodies to B.gibsoni in dogs.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Parasitology and Parasitic Diseases
Year:
2006
Type:
Article