Detection of Chromosome-encoded ?-Lactamase Gene Families in Klebsiella pneumoniae by Using Nested Polymerase Chain Reaction (nPCR) / 中华医院感染学杂志
Chinese Journal of Nosocomiology
;
(24)1994.
Article
in Chinese
| WPRIM
| ID: wpr-588209
ABSTRACT
OBJECTIVE To survey the chromosome-encoded ?-lactamase gene families SHV,LEN and OKP in(continuously) isolated Klebsiella pneumoniae in China.METHODS The existence and statistical distribution of all three gene families in antibiotic resistant K.pneumoniae strains were detected by applying nPCR.RESULTS In 44 strains,28 strains were SHV gene positive(63.6%),4 strains were LEN gene positive(9.1%) and none in OKP gene.CONCLUSIONS All the three gene families are chromosome-encoded and have 80-92% of genetic(homogeneity),so they all could identifiy the most important factors assisting the spread of bacterial drug (resistance).nPCR is an economic and convenient measurement to detect the chromosome-encoded ?-lactamase gene.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Nosocomiology
Year:
1994
Type:
Article
Similar
MEDLINE
...
LILACS
LIS