Quantification of IL-10 gene expression in induced sputum of asthmatic patients by competitive PCR / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2004.
Article
in Chinese
| WPRIM
| ID: wpr-588224
ABSTRACT
Objective:
To investigate the role of anti-inflammatory cytokine IL-10 in the pathogenesis of asthma.Methods:
IL-10 internal standards was constructed by restriction digest.The expression levels of IL-10 mRNA were determined in peripheral blood and induced sputum of 23 asthmatic patients by(RT-PCR) with this competitive internal standards.Results:
There was no IL-10 mRNA expression in most of the asthmatics.IL-10 mRNA was detected in some catabatic patients.But IL-10 mRNA was readily detected in 29 normal subjects.Conclusion:
Airway inflammation of asthma was related to the expression of IL-10 gene.Asthmatic patients may have difficulty in IL-10 synthesis with transcript.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Medical Postgraduates
Year:
2004
Type:
Article
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