Enrichment of dendritic cells precursor from chronic myelogeous leukemia by Percoll density gradients / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition)
;
(6)2006.
Article
in Chinese
| WPRIM
| ID: wpr-588267
ABSTRACT
Objective To set up an effective and low-price way for enriching dendritic cells precursor from chronic myelogeous leukemia by Percoll density gradients.Methods Peripheral blood mononuclear cells(PBMCs) were collected by separation through Ficoll-Hypaque,then PBMCs were separated by 55% Percoll gradients. The cell type and DCs expressing CD1a,CD86 were detected in the high-density group(C),low-density group(B) and non-Percoll separation group(A).Results After separation of 55% Percoll,the percentage of promyelocytes and myelocytes in group B was obviously higher than those in group A and group C(P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Jilin University(Medicine Edition)
Year:
2006
Type:
Article
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