Primary culture of nontumorigenic human mammary epithelial cells / 基础医学与临床

ABSTRACT

Objective To investigate the feasibility of primary culture of nontumorigenic human mammary epithelial cells,and the effect of proliferation due to hormone.Methods Normal human mammary tissue was taken from mammary segment ectomy,and the tissue was digested by collagenase type Ⅰ.Epithelial depuration was carried out by integration of burning and enzyme digestion.The cells we acquired were identified by observation of cell morphology by light and Transmission Electron Microscopy(TEM).Results It is feasible to culture nontumorigenic human mammary epithelial cells.?-estradiol and progesterone at 1?10-5 g/L can promote the proliferation of the normal human mammary epithelial cells.