Influences of basic fibroblast growth factor and epidermal growth factor on the proliferation and differentiation of neural stem cells / 中国组织工程研究

ABSTRACT

OBJECTIVE: How to promote the generation of neural stem cells (NSCs) and induce them differentiate into the goal cell type has been the focus of neuroscience. In this article, we review the adjustment of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) to the proliferation and differentiation of NSCs. DATA SOURCES A computer-based online search of Medline database was undertaken to identify the related articles published in English from January 1998 to March 2006 by using the keywords of "bFGF, EGF, nerve stem cells". At the same time, Chinese relevant articles were searched in China National Knowledge Infrastruction published between January 2000 and March 2006 with the same keywords in Chinese. STUDY SELECTION The data were selected firstly to look over the citations of every literature. Inclusive criteria Articles related to the effect of bFGF and EGF on the proliferation and differentiation of NSCs. Exclusive criteria Duplicate research or Meta analysis. DATA EXTRACTION A total of 78 related articles were collected, of which 32 accorded with the inclusive criteria and 46 with duplicate or old contents literatures were eliminated. Among the 32 literatures, 8 involved in the research status of NSCs, 10 about the effect of bFGF on the proliferation and differentiation of NSCs and the mechanism, 4 involved in the effect of EGF on the proliferation and differentiation of NSCs and the mechanism, respectively, 8 related to the effect of bFGF and EGF together, and 5 related to the comparison of the effect of bFGF and EGF. DATA SYNTHESIS: ①NSCs are self-renewed, and can differentiate into different stem cells and mature tissue cells in different microenvironment. Many cytokines in the microenvironment play essential roles in the differentiation direction of NSCs. ②bFGF has strong improvement effect on the proliferation of NSCs and can activate the potential regeneration capacity of neuronal precursor cell in distinct areas of central nervous system, proliferation and differentiation of cells present bFGF concentration dependent. ③EGF can improve the growth of NSCs, and promote them differentiate into neuron and glial cells; its effect of facilitating cell proliferation also presents concentration dependent. ④To elevate the efficacy of proliferation and differentiation of NSCs, allied application of bFGF and EGF is usual in NSCs culture. ⑤There is not a unified cognition to the effect difference of bFGF and EGF. CONCLUSION: bFGF and EGF have powerful effect to improve the proliferation and differentiation of NSCs; therefore, both of them are important neural somatomedin. Moreover, their effects present concentration dependent.