Influence of nerve growth factor on the expression of nestin and neural stem cell type in rats with focal cerebral ischemia / 中国组织工程研究

ABSTRACT

AIM: To investigate the effect of nerve growth factor (NGF) on the proliferation of neural stem cell in adult rats with focal cerebral ischemia. METHODS: The experiment was conducted in the Center of Science and Experiment, Jinzhou Medical College from January to July 2006. Totally 72 healthy SD rats were randomly divided into sham-operation group, model group and NGF treatment group with 24 rats in each group. Focal cerebral ischemia models were prepared by Logna et al modified thread occlusion method, and the functional evaluation was performed after the animals were awake for 2 hours to select the rats whose nervous function reached grade 2 or above. The sham-operation group was treated like the model group except thread occlusion. 1 000 ?g/kg NGF was administered in the NGF treatment group immediately after ischemia, once a day. After the animals were executed at days 1, 3, 7 and 14 days, immunohistochemical and double immunofluorescence labeling were adopted to observe the effect of NGF on the expression of nestin and the cell type after ischemia. RESULTS: All 72 rats were involved in the result analysis. ①Round or ellipse nestin positive cells were found in both the model group and NGF treatment group. The number of nestin positive cells in the NGF treatment group was remarkably more than in model group except 1 day after ischemia, moreover, the number of the cells in the two groups was more than in the sham operation group [model group (3.47?0.5), (5.13?1.14), (13.95?3.56), (8.97?2.08); NGF group (3.81?0.66), (9.88?2.08), (19.87?3.86), (26.17?2.90); sham-operation group 0, P