Neuroprotective effect of calcitriol in systemic inflammation induced by lipopolysaccharide / 医学研究生学报

ABSTRACT

Objective:To investigate neuroprotection of calcitriol in LPS-induced rat systemic inflammation and underlying mechanism. Methods:Adult male Sprague-Dawley rats were randomized into three groups blank group(6 rats),calcitriol group [23 rats,4?g/(kg?d)?14d] and control group [23 rats,fat milk,4ml/(kg?d)?14d].46 rats were induced systemic inflammation by intraperitoneal injection of LPS(10mg/kg) on day 14th,1 hour after the administration.On post-injection 3,6,9 hour,36 rats were anesthetized to death and obtained rat brain tissue to detect activity of NF-?B by EMSA and to assess TNF-?,IL-10 concentration by ELISA.HE staining was used to determine brain injury. Results:LPS effectively activated NF-?B,enhanced TNF-? expression,induced sporadic neurons damage in brain in the control group and in time-dependent,compared with blank group rats.Calcitriol significantly down-regulated NF-?B activity,TNF-? expression but enhanced IL-10 expression at all time point evaluated,compared with the control group rats. Conclusion:Intraperitoneal injection of LPS can induce brain damage,Calcitriol modulate the inflammatory-antiinflammatory cytokine balance by downregulating activation of NF-?B,reducing over express of TNF-? and elevating IL-10 concentration.